Journal: Genes & nutrition

Article Title: Protective effects of Nogo-B deficiency in NAFLD mice and its multiomics analysis of gut microbiology and metabolism.

doi: 10.1186/s12263-024-00754-5

Figure Lengend Snippet: Fig. 2 Nogo-B deficiency alleviates weight gain and impaired glucose tolerance in NAFLD model mice. (A) Photos of the mice and their livers at the end of the 12-week experiment; (B) body weight (weekly); (C) body weight (last measurement); (D) liver weight (last measurement); (E) glucose tolerance test (GTT); (F) AUC of the GTT; (G) insulin tolerance test (ITT); (H) AUC of the ITT. n = 6. #P < 0.05, ##P < 0.01, ###P < 0.001; *P < 0.05, **P < 0.01, ***P < 0.001. NCD, normal chow diet; HFD, high-fat diet; AUC, area under the curve; BMDMs, bone marrow-derived macrophages; WT, wild-type; NCD, normal chow diet; HFD, high-fat diet

Article Snippet: Following an 8-week period of standard growth, 12 WT mice and 12 Nogo-B−/− mice were randomly divided into the normal chow diet (NCD) or high-fat diet (HFD) groups, which were reared in a barrier system at temperatures ranging from 20 to 26 °C and fed either the NCD (SPF (Beijing) Biotechnology Co., Ltd.) or HFD (fat diet with 60 kcal%, MP Biomedicals, LLC) for 12 weeks.

Techniques: Derivative Assay

Journal: Genes & nutrition

Article Title: Protective effects of Nogo-B deficiency in NAFLD mice and its multiomics analysis of gut microbiology and metabolism.

doi: 10.1186/s12263-024-00754-5

Figure Lengend Snippet: Fig. 3 Nogo-B deficiency mitigates liver lipid accumulation and liver injury in NAFLD model mice. (A) Serum triacylglycerol (TG); (B) serum total cholester ol (TC); (C) serum low-density lipoprotein cholesterol (LDL-C); (D) serum high-density lipoprotein cholesterol (HDL-C); (E) serum alanine aminotransferase (ALT); (F) serum aspartate aminotransferase (AST); (G) serum alkaline phosphatase (ALP); (H) serum albumin (ALB); (I) hematoxylin and eosin (H&E) stain ing of liver sections (scale bar: 200 μm); (J) oil red O staining of liver sections (scale bar: 200 μm). n = 6. #P < 0.05, ##P < 0.01, ###P < 0.001; *P < 0.05, **P < 0.01, ***P < 0.001. WT, wild-type; NCD, normal chow diet; HFD, high-fat diet

Article Snippet: Following an 8-week period of standard growth, 12 WT mice and 12 Nogo-B−/− mice were randomly divided into the normal chow diet (NCD) or high-fat diet (HFD) groups, which were reared in a barrier system at temperatures ranging from 20 to 26 °C and fed either the NCD (SPF (Beijing) Biotechnology Co., Ltd.) or HFD (fat diet with 60 kcal%, MP Biomedicals, LLC) for 12 weeks.

Techniques: Staining

Journal: Genes & nutrition

Article Title: Protective effects of Nogo-B deficiency in NAFLD mice and its multiomics analysis of gut microbiology and metabolism.

doi: 10.1186/s12263-024-00754-5

Figure Lengend Snippet: Fig. 4 Nogo-B deficiency attenuates inflammation in the livers of NAFLD model mice. (A) The concentration of interleukin-6 (IL-6); (B) the concentration of tumor necrosis factor-α (TNF-α); (C) the mRNA expression of tumor necrosis factor-α (TNF-α); (D) the mRNA expression of monocyte chemoattractant protein-1 (MCP1); (E) the mRNA expression of CD80; (F) the mRNA expression of CD86; (G) the mRNA expression of arginase-1 (Arg1); (H) the mRNA ex pression of arginase-1 (Arg1); (I) the mRNA expression of macrophage galactose N-acetyl-galactosamine specific lectin-2 (Mgl2); (J) the mRNA expression of resistin-like alpha (Retlna). n = 6. #P < 0.05, ##P < 0.01, ###P < 0.001; *P < 0.05, **P < 0.01, ***P < 0.001. WT, wild-type; NCD, normal chow diet; HFD, high-fat diet

Article Snippet: Following an 8-week period of standard growth, 12 WT mice and 12 Nogo-B−/− mice were randomly divided into the normal chow diet (NCD) or high-fat diet (HFD) groups, which were reared in a barrier system at temperatures ranging from 20 to 26 °C and fed either the NCD (SPF (Beijing) Biotechnology Co., Ltd.) or HFD (fat diet with 60 kcal%, MP Biomedicals, LLC) for 12 weeks.

Techniques: Concentration Assay, Expressing

Journal: Genes & nutrition

Article Title: Protective effects of Nogo-B deficiency in NAFLD mice and its multiomics analysis of gut microbiology and metabolism.

doi: 10.1186/s12263-024-00754-5

Figure Lengend Snippet: Fig. 6 Nogo-B deficiency regulates serum metabolites in NAFLD model mice. (A) Principal component analysis (PCA) score plot; (B) partial least squares discriminant analysis (PLS-DA) score plot; (C) orthogonal partial least squares discriminant analysis (OPLS-DA) score plot; (D) OPLS-DA permutation test; (E) volcano plot, in which the red spots represent the significantly upregulated metabolites (log2FC > 0), the blue spots represent the significantly down regulated metabolites (log2FC < 0), and the gray spots represent the nonsignificant metabolites (P > 0.05); (F) clustered heatmap and VIP value bar graphs of differentially abundant metabolites (top 30 VIP values) between the WT-HFD and Nogo-B−/−-HDF groups; (G) KEGG pathways (top 20 enrichment ra tios) of differentially abundant metabolites. n = 5. VIP, variable importance in the projection; WT, wild-type mice; NCD, normal chow diet; HFD, high-fat diet

Article Snippet: Following an 8-week period of standard growth, 12 WT mice and 12 Nogo-B−/− mice were randomly divided into the normal chow diet (NCD) or high-fat diet (HFD) groups, which were reared in a barrier system at temperatures ranging from 20 to 26 °C and fed either the NCD (SPF (Beijing) Biotechnology Co., Ltd.) or HFD (fat diet with 60 kcal%, MP Biomedicals, LLC) for 12 weeks.

Techniques: